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Development and Validation of Assay Method for the Estimation of Moxifloxacin in Bulk and Pharmaceutical Formulations by RP-HPLC

1,2, 1,2, 3, 1

  1. 1Department of Chemistry, Federal Urdu University of Arts, Sciences and Technology, Gulshan-e-Iqbal, Karachi, PAKISTAN..
  2. 2School of Chemical and Biomolecular Sciences, Lincoln Dr, Southern Illinois University at Carbondale, USA..
  3. 3Department of Pharmacy, Federal Urdu University of Arts, Sciences and Technology, Gulshan-e-Iqbal, Karachi, PAKISTAN..

CORRESPONDENCE

Zahid Siraj

Department of Chemistry, Federal Urdu University of Arts, Sciences and Technology, Gulshan-e-Iqbal, Karachi, PAKISTAN..

zahid.siraj@siu.edu

Received: 05-08-2024; Accepted: 04-09-2024.

Volume 18, Issue 1 · pp. 64-70 · 2024 · DOI: 10.5530/ijopp.20250084

ABSTRACT

Background: A simple, specific andisocratic reversed phase-high performance liquid chromatography (RP-HPLC) method with UV detection at 293 nm and column Agela Technology, C18, (Venosil XBP, 4.6 mm×250 mm, 10 μm) was developed and validated for analysis of Moxifloxacin hydrochloride (MOXI) in presence of its degradation products. The method was validated in accordance with International Conference on Harmonization (ICH) guidelines.Materials and Methods:The study used a mobile phase consisting of a phosphate buffer and methanol (18:7 v/v), with a flow rate of 1.3 mL/min. The temperature was maintained at 50ºC using a column oven. Additionally, 0.1N HCl was used as the diluent.Results:The retention time of the MOXI was found at 9.99 min. The calibration curves were linear with correlation coefficient (R2) of 0.999. The detection and quantification limit were found as 0.029 μg mL-1and 0.095 μg mL-1respectively.Conclusion:The proposed method was found to be sensitive, specific and was successfully applied for the estimation of MOXI in pharmaceutical formulations. Innumerable analytical measurements are conducted for the estimation of MOXI in pharmaceutical formulations, so the proposed analytical method leads to provision of cost effective by using low-cost diluents and reagents.

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    Siraj, Z., Adil, O., Amin, B., & Tahiri, I. A. (2024). Development and Validation of Assay Method for the Estimation of Moxifloxacin in Bulk and Pharmaceutical Formulations by RP-HPLC. Indian Journal of Pharmacy Practice, 18(1), 64-70. https://doi.org/10.5530/ijopp.20250084

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    © 2024 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. CC BY 4.0